Figure 4.

FBEVs modulates intracellular pathways in MMECs. MMECs were cultured alone or co-cultured with FBEVs (n = 3 patients) for (A,C) 6 h or (B–E) 24 h. (A,B) Cell lysates were analyzed using a phospho-kinase array. (C,D) VEGFA, IL-6 and IL-8 mRNA levels were analyzed byRT-qPCR. mRNA fold-expression was normalized to endogenous GAPDH and expressed as mean ± S.D. vs. MMECs. (E) Conditioned media of MMECs cultured alone or co-cultured with FBEVs for 24 h was tested for angiogenic cytokines using an angiogenesis array. Data are normalized to reference spots and expressed as mean protein levels ± S.D. * p < 0.05.