Table 2.
Translation and selection of top markers into qPCR format.
| Episwitch Interaction a | Primers Design ID b | Probe Used | Opt Ann Tm c | QC Optimisation d | Screen 1 | Screen 2 |
|---|---|---|---|---|---|---|
| CASP6_4_109703339_109741090_RF | OBD189-q005/q007 | OBD189-p005 | 68 | Passed | Passed | Passed |
| IGF1R_15_98731539_98790114_FF | OBD189-q001/q003 | OBD189-p003 | 64.4 | Passed | Failed | |
| IKBKB_8_42264241_42292124_FR | OBD189-q025/q027 | OBD189-p025 | 66.4 | Passed | Failed | |
| IKBKB_8_42264241_42332799_FR | OBD148_261/263 | OBD189-p261 | 68 | Passed | Failed | |
| IL17D_13_20664875_20691044_FF | OBD189-q077/q079 | OBD189-p077 | 67.5 | Passed | Failed | |
| ITK_5_157178319_157271762_RR | OBD189-q065/q067 | OBD189-p065 | 66.4 | Passed | Passed | Passed |
| MYC_8_127691489_127740424_FR | OBD189-q013/q015 | OBD189-p013 | 66.4 | Passed | Failed | |
| ORF102_17_34316073_34373948_RF | OBD148-q893/q895 | OBD148-p893 | 62 | Passed | Passed | Passed |
| ORF197_8_26561792_26644530_FR | OBD189-q069/q071 | N/A e | N/A | Failed | Failed | |
| ORF243_1_161633494_161661864_RF | OBD189-q041/q043 | OBD189-p043 | 62 | Passed | Failed | |
| ORF313_13_20664875_20698635_FF | OBD189-q081/q083 | OBD189-p081 | 64.4 | Passed | Passed | Passed |
| ORF313_13_20664875_20744490_FR | OBD189-q073/q075 | N/A | N/A | Failed | Failed | |
| ORF369_13_46087370_46193039_RF | OBD189-q053/q055 | OBD189-p053 | 67.5 | Passed | Passed | Passed |
| ORF479_8_81007411_81099880_FR | OBD189-q061/q063 | OBD189-p061 | 64.4 | Passed | Failed | |
| ORF480_11_77430379_77519103_RF | OBD189-q033/q035 | OBD189-p033 | 66.4 | Passed | Failed | |
| ORF482_5_168579937_168620163_RR | OBD189-q021/q023 | OBD189-p021 | 66.4 | Passed | Failed | |
| ORF698_18_62296384_62386748_FF | OBD189-q045/q047 | OBD189-p045 | 66.4 | Passed | Failed | |
| ORF698_18_62330039_62362521_FR | OBD189-q037/q039 | N/A | N/A | Failed | Failed | |
| ORF703_1_6461604_6515315_FR | OBD189-q029/q031 | OBD189-p031 | 67.5 | Passed | Passed | Passed |
| ORF705_9_114855753_114929419_FR | OBD189-q049/q051 | OBD189-p049 | 66.4 | Passed | Passed | Passed |
| ORF712_9_120888366_120919710_RR | OBD189-q017/q019 | OBD189-p017 | 68 | Passed | Failed | |
| PDCD1LG2_9_5495992_5572986_RR | OBD189-q057/q059 | OBD189-p057 | 67.5 | Passed | Passed | Passed |
| TNFSF8_9_114957908_114977746_RF | OBD148-q917/q919 | N/A | N/A | Failed | Failed | |
| TRAF2_9_136904007_136941363_RF | OBD189-q009/q011 | OBD189-p009 | 64.4 | Passed | Failed |
a Internal EpiSwitch Interaction ID. b Internal primer design ID. c Optimum Annealing Temperature. d Quality Control Optimisation. e Not Available. Temperature gradient optimisation for primer probe designs of the top 24 markers: markers that failed quality control and feature reduction in Screen 1 and Screen 2, are marked.