Figure 9.
The efficiency of plating for selected phages in MAB. (A) The EOP was evaluated by a 10-fold serial dilution of phage lysates and spotting 3 μL of the 10−1 to 10−8 dilutions onto top agar overlays of M. smegmatis mc2155 or MAB 19977. (B) The degree of phage virulence was recorded for selected 17 phages as a ratio between the titer of the phage at the terminal dilution on MAB divided by the titer of the same phage on M. smegmatis. On this basis, phages were classified as highly virulent (0.1 < EOP < 1.00), moderately virulent (0.001 < EOP < 0.09), or low virulence (EOP < 0.009).