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Structure evolution from the most frequent variant of lineage L1 PE_PGRS33all29 (top) to PE_PGRS33AllRv (bottom). The inset in the top panel reports the superposition of GRLPI motifs of the two predicted structures; similar backbone torsion angles are observed for Pro116 and Leu116 (φ = −70, ψ = 150). In the bottom panel, inserted regions from PE_PGRS33all29 to PE_PGRS33Rv variants are drawn in dark blue.
