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Titration of the amount of nuclear GST-M protein required for M activity. Export of 32P-labeled U1Sm− snRNA, AdML mRNA and tRNA was analyzed in oocytes preinjected 20 min earlier in the nucleus with 1,500 pg of recombinant GST protein (a) and with the indicated amounts (b to d) of GST-M protein. RNA export was monitored 1 and 3 h after injection of export substrates. The asterisk indicates a degradation product of U3 snoRNA.
