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. 2023 Apr 24;13(5):730. doi: 10.3390/biom13050730

Table 3.

Yields (% rel. quant.) of 18:1 (oleic acid), 18:2 (linoleic acid), and Ara and their corresponding monotrans (mt) isomers obtained after transesterification of RBC ghosts (1 mM) incubated at 37 °C in the open air for 15 h in the presence of 10 µM Fe2+, 100 µM H2O2, with/without the addition of 10 μM 2-mercaptoethanol as a thiol.

Thiol (μM) 9cis-18:1 9trans-18:1 Ara Ara Loss mtAra 18:2 18:2 Loss mt-18:2
0 99.0 ± 1.0 - 66.9 ± 1.1 33.1 ± 1.1 - 73.4 ± 2.1 26.5 ± 2.1 -
10 99.2 ± 0.1 0.7 ± 0.1 95.0 ± 0.2 2.3 ± 0.01 2.3 ± 0.2 96.1 ± 0.8 2.5 ± 0.6 1.4 ± 0.2

Values are means of n = 3 experiments under the same conditions expressed as a relative percentage (% rel. quant.) of each fatty acid isomer with respect to the starting cis fatty acid residue, estimated by GC analysis using calibration and 17:0 as internal standard; Yields of the three repetitions were found with errors <0.05%.