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. 2023 May 13;12(10):1380. doi: 10.3390/cells12101380

Figure 1.

Figure 1

NLS-TdTom is only inside the nucleus during interphase. Selected time points of Video S2. NLS-TdTom (magenta) diffuses over the whole cell upon permeabilization of the nucleus at the onset of mitosis (60 s, arrow). Cells co-expressing GFP-α-tubulin (green) are observed to show the mitotic progression. The nuclear marker is diffused completely before the centrosome duplication (arrowhead) becomes visible (310 s). After cytokinesis, NLS-TdTom is located back to the nucleus (1110 s). Bar 5 µm.