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. 2023 May 13;12(10):1380. doi: 10.3390/cells12101380

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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NE permeabilization versus Nup62 disassembly during mitosis. (A) Selected time points from live-cell imaging (Video S10) of a strain co-expressing the Nup62-Neon knock-in construct (green) and the NE permeabilization marker NLS-TdTom (magenta). NLS-TdTom leaves the nucleus at the onset of mitosis while Nup62-Neon is still present at the NE (arrow at 20 s). Nup62-Neon completely disassembles from the NE (arrow at 80 s) before it reappears in metaphase (arrow at 410 s). Out of a 12-layer image stack (z-distance 0.5 µm), only the focus plane is presented, respectively. Images were recorded with a 10 s time lapse. Bar 5 µm. (B) The graph presents the fluorescence intensity values for Nup62-Neon knock-in and NLS-TdTom over the complete cell division in (A).