The altered connected subnetwork in patients with ALS based on the NBS analysis. (A) Altered functional network connectivity in ALS. A single connected subnetwork containing 39 nodes with 49 significantly increased connections in patients with ALS compared with the HC group (p = 0.019, corrected). (B) Altered grey matter structural network connectivity in ALS. ALS patients showed significantly increased connections within the first subnetwork consisting of 129 nodes and 202 edges (p = 0.009, corrected, the upper row of (B)). The second subnetwork was composed of 73 nodes and 168 edges, which was significantly decreased in ALS compared with the HC group (p = 0.009, corrected, the bottom row of (B)). Abbreviations: ALS, amyotrophic lateral sclerosis; HC, healthy control; NBS, network-based statistic; SN, subcortical network; LN, limbic network; VN, visual network; SMN, somatomotor network; FPN, frontoparietal network; DMN, default mode network; DAN, dorsal attention network; SVAN, salience/ventral attention network.