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. 2023 May 1;13(5):784. doi: 10.3390/biom13050784

Figure 5.

Figure 5

Tubulin polyglutamylation and neurofilaments in dendrites of Ttll knockout mice. (A) Cortical brain sections from Ttll knockout mice were stained with antibodies against glutamylated tubulin (left panels, green, E- α/β Tub), MAP2 (middle panels, red), and Hoechst 33342 for nucleus imaging (right panels, blue). (B) Cortical brain sections from Ttll knockout mice were stained with antibodies against neurofilament M (left panels, green), MAP2 (middle panels, red), and Hoechst 33342 for nucleus imaging (right panels, blue). Scale bars: 20 µm. (C) Immunoblot analysis of neurofilaments proteins stained by antibodies against neurofilament (NF-H and NF-M) and GAPDH as a control. (D) Immunoblot analysis of MAPs and motor proteins co-sedimented with brain tubulin from Ttll knockout mice. Abbreviations: 1KO: Ttll1 knockout; 7KO: Ttll7 knockout; dKO: Ttll1/Ttll7 double knockout; E-α/βTub: polyglutamylated α/β-tubulin.