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. 2023 May 1;13(5):784. doi: 10.3390/biom13050784

Figure 6.

Figure 6

Quantitative analyses of the mice brain glutamate by LC-MS/MS. (A) Standard curve of glutamate in the LC-MS/MS quantitative analysis. (B) Quantitative analysis Ttll knockout mice half brain coronal sections glutamate concentration by an Acquity UPLC system with 4000 QTRAP linear ion trap quadrupole mass spectrometer. (WT: n = 3 mice; 1KO: n = 3 mice; 7KO: n = 3 mice; dKO: n = 3). (C) Using neutralized extraction solutions extract Ttll7 knockout and WT mice brain glutamate, quantitative analysis by 4000 QTRAP mass spectrometer, unpaired t test. (WT: n = 3 mice; 7KO: n = 3 mice). (D) Quantitative analysis Ttll7 knockout and WT mice brain DPP-Glu by 4000 QTRAP mass spectrometer, unpaired t test. (WT: n = 3 mice; 7KO: n = 3 mice). (E) Quantitative analysis Ttll7 knockout and WT mice whole brain coronal sections glutamate detected by Xevo TQ-XS triple quadrupole mass spectrometer, unpaired t test. (WT: n = 6 mice; 7KO: n = 6 mice). All data represent as mean ± SD, unpaired t test. Abbreviations: 7KO: Ttll7 knockout.