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. 2023 May 20;12(10):1433. doi: 10.3390/cells12101433

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Time-dependent expression of IDO2 after treatment with TCDD in wildtype and AhR-knockout MCF-7 cells. Cells were treated with 1 nM TCDD for various time points for 3, 6, 12, 24, and 48 h and then harvested for RNA extraction and IDO2 expression analysis via qPCR after 24 h treatment. TCDD stock solution was dissolved in DMSO and control cells received 0.1% DMSO. The expression was corrected against the housekeeping gene ß-actin. Results are presented as mean ± SEM, and the y-axis represents mRNA expression level. The expression level of IDO2 mRNA in control cells is shown in dotted lines; * significantly higher than the control (p < 0.05).