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. 2023 May 20;12(10):1433. doi: 10.3390/cells12101433

Figure 2.

Figure 2

Effect of AhR ligands on the expression of IDO1 and IDO2 in MCF-7 cells. MCF-7 cells were treated with FICZ (100 nM), Kyn (50 µM), TCDD (1 nM), BaP (2.5 µM), and TRAP PM (10 µg/mL) for 24 h. In addition, cells were treated with 100 U/mL IFNγ (data not shown), which induced a 245-fold increase in IDO1 but had no effect on IDO2 expression. Cells were harvested for RNA extraction and (A) IDO2 and (B) IDO1 expression analysis via qPCR after 24 h treatment. The expression was corrected against the housekeeping gene ß-actin. Results are presented as mean ± SD, and the y-axis represents the mRNA expression level; * significantly higher than control (p < 0.05).