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. 2023 May 20;12(10):1433. doi: 10.3390/cells12101433

Figure 3.

Figure 3

TCDD-induced IDO2 promoter activity is AhR- and XRE-dependent: (A) Schematic illustration of the promoter construct of the mouse ido2 gene containing 3275 bp upstream of the transcriptional start site cloned into a luciferase (luc) reporter vector. The positions of the short-tandem repeat (STR) at −2478 bp containing four putative XRE consensus sequences, two XRE consensus sites at −1949 bp and −1400 bp, and one recognition site for ISRE at −1333 bp are shown. (B) Wildtype (WT) and AhR-knockout (AhR−/−) MCF-7 cells were transfected with the IDO2 luciferase reporter construct containing 3275 bp of the human ido2 gene promoter region; a, significantly higher than WT control cells (p < 0.05); b, significantly lower than treated WT cells. (C) MCF-7 WT cells were transfected with the full-length (3275 bp) IDO2 reporter plasmid, a −2325 bp deletion construct, and a 432 bp (Δ−2563-2131) construct containing the STR sequence with four XRE core elements. Cells were transfected for 16 h and treated with 1 nM TCDD or 100 U/mL IFNγ for 6 h. Relative luciferase activity units are given as mean values of triplicates as a result of three independent experiments; *, significantly different from control cells (p < 0.05).