Figure 4.

ChIP assay for STR region of the human IDO2 promoter. TCDD induces the recruitment of AhR to the STR region. MCF-7 WT cells were treated with 1 nM TCDD or 100 U/mL IFNγ, for 6 h, and anti-AhR antibodies were used for immunoprecipitation. Data show the ratio of the enrichment of the promoter region target in TCDD-treated cells. The increase in the enrichment was calculated relative to that of the negative control with anti-IgG. qPCR was performed to analyze the levels of AhR binding to the STR on the IDO2 promoter. Error bars indicate standard deviations from the mean of at least three experiments; * significantly higher than control (p < 0.05).