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. Author manuscript; available in PMC: 2024 Mar 30.
Published in final edited form as: Cell. 2023 Mar 22;186(7):1382–1397.e21. doi: 10.1016/j.cell.2023.02.022

Figure 5. CCHa1 regulates arousability through dopaminergic neurons in the brain.

Figure 5.

(A) Arousabilty from sleep when the CCHa1 receptor is depleted with various Gal4 drivers. (B) Schematized dopaminergic neurons in the fly brain, and narrowing down of the population to which CCHa1 signals. (C) PAMMB441B-Gal4 expression in the brain is visualized with membrane-targeted GFP. Presynaptic protein Brp marks the neuropil, allowing visualization of the mushroom body (dashed outline). Scale bar: 100 μm. Arousabilty from sleep when the CCHa1 receptor is depleted from PAMMB441B neurons (PAMMB441B>CCHa1-RNAi). (D) Arousability from sleep when PAMMB441B neurons are inhibited (green) or activated (yellow). (E) Arousability from sleep when tyrosine hydroxylase (TH) is depleted from PAMMB441B neurons (PAMMB441B>TH-RNAi). (F) Schematic summary. Error bars, mean and S.E.M. Genotypes, sample sizes and statistical analyses, Table S3. See also Figure S5.