Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| rabbit anti-CCHa1 | This study | N/A |
| mouse anti-Bruchpilot | DHSB (Developmental Studies Hybridoma Bank) |
Cat# nc82; RRID: AB_2314866 |
| chicken anti-GFP | Aves | Cat# GFP-1020; RRID: AB_10000240 |
| mouse anti-prospero | DSHB | Cat# pros; RRID: AB 528440 |
| mouse anti-tyrosine hydroxylase | ImmunoStar | Cat# 22941 ; RRID: AB 572268 |
| Rabbit anti-β-galactosidase | MP Biomedicals | Cat#56033 LOT#06026 |
| Alexa Fluor 488 goat anti-chicken | Life technologies | Cat# A11039; RRID: AB_142924 |
| Alexa Fluor 488 goat anti-rabbit | Life technologies | Cat# A11034; RRID: AB 2576217 |
| Alexa Fluor 647 goat anti-rabbit | Life technologies | Cat# #A21244; RRID: AB 2535812 |
| Alexa Fluor 647 donkey anti-mouse | Life technologies | Cat# A31571; RRID: AB_162542 |
| Bacterial and virus strains | ||
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| Coconut oil | Spectrum essentials | Cat# L215562P-006 |
| Propionic acid | Sigma-Aldrich | Cat# P1386; CAS: 79–09-4 |
| Hexanoic acid | Sigma-Aldrich | Cat# P21530; CAS 142–62-1 |
| Glucose | Sigma-Aldrich | Cat# 158968–1KG; CAS: 492–62-6 |
| Fructose | Sigma-Aldrich | Cat# F0127; CAS:57–48-7 |
| Galactose | Sigma-Aldrich | Cat# G0750; CAS: 59–23-4 |
| Peptone | Apex | Cat# 20–260 |
| Single amino acids | Sigma-Aldrich | Cat# LAA21–1KT |
| FD&C Blue #1 | Spectrum | Cat# FD110; CAS: 3844–45-9 |
| Triton X-100 | Amresco | Cat# M143; CAS: 9002–93-1 |
| PBS 10x | SeraCare | Cat# 5460–0030 |
| Ethanol (pure) | Koptic | Cat# V1001; CAS: 64–17-5 |
| PFA (16%) | Electron Microscopy Sciences | Cat# 15710; CAS: 30525–89-4 |
| BSA | Gemini | Cat# 700–101P; CAS: 9048–46-8 |
| Microscope glass slides | Electron Microscopy Sciences | Cat# 63421–10 |
| Microscope coverslips | Electron Microscopy Sciences | Cat# 72230–01 |
| Prolong Gold Antifade medium | Invitrogen | Cat# 1942345 |
| TRIzol | Thermo fisher | Cat# 15596026 |
| iScript cDNA Synthesis | Biorad | Cat# 1708890 |
| Sybr Green | Biorad | Cat# 170–8880 |
| Critical commercial assays | ||
| Direct-zol RNA Microprep | Zymo Research | Cat# R2061 |
| DNAse Turbo DNA-free kit | Thermo fisher | Cat# AM1907 |
| Deposited data | ||
| Experimental models: Cell lines | ||
| Experimental models: Organisms/strains | ||
| wild type iso31 | Gift from Kyunghee Koh | N/A |
| elav-Gal4 | Gift from Kyunghee Koh | N/A |
| UAS-TrpA1 | Gift from Kyunghee Koh | N/A |
| UAS-Shits | Gift from Kyunghee Koh | N/A |
| elav-Gal4;UAS-Dcr2 | Gift from Nicholas Stavropoulos | N/A |
| PAM-Gal4 | BDSC (Bloomington Drosophila Stock Center) | RRID: BDSC_41347 |
| UAS-CCHa1-RNAi | BDSC | RRID: BDSC_57562 |
| UAS-CCHa1-RNAi #2 | VDRC (Vienna Drosophila Recourse Center) | RRID:SCR_013805 #104074 |
| UAS-CCHa1R-RNAi | BDSC | RRID: BDSC_51168 |
| UAS-CCHa1R-RNAi #2 | VDRC | RRID:SCR_013805 #103055 |
| UAS-GFP-RNAi | BDSC | RRID: BDSC_41552 |
| UAS-DopR1-RNAi | BDSC | RRID: BDSC_31765 |
| UAS-DopR2-RNAi | BDSC | RRID: BDSC_65997 |
| UAS-D2R-RNAi | BDSC | RRID: BDSC_36824 |
| UAS-DopEcR-RNAi | BDSC | RRID: BDSC_31981 |
| Tdc2-Gal4 | BDSC | RRID: BDSC_9313 |
| vGlut-Gal4 | BDSC | RRID: BDSC_26160 |
| PAMMB441B | BDSC | RRID: BDSC_68251 |
| MBONMB083C | BDSC | RRID: BDSC_68287 |
| CaLexA | BDSC | RRID: BDSC_66542 |
| UAS-nlsGFP | BDSC | RRID: BDSC_4776 |
| UAS-TH-RNAi | BDSC | RRID: BDSC_25796 |
| UAS-Dcr2 | BDSC | RRID: BDSC_24650 |
| CCHa1 mutant Mi{MIC}CCHa1MI09190 | BDSC | RRID: BDSC_51261 |
| CCHa1R mutant Mi{MIC}CCHa1RMI08118 | BDSC | RRID: BDSC_44750 |
| TI{2A-GAL4}CCHa1-R[2A-GAL4] | BDSC | RRID: BDSC_84601 |
| P{GMR23H07-GAL4}attP2 | BDSC | RRID: BDSC_47498 |
| UAS-CD8GFP | Gift from Michael Crickmore | N/A |
| UAS-nlsLacZ | Gift from Michael Crickmore | N/A |
| TH-Gal4 | Gift from Michael Crickmore | N/A |
| ddc-Gal4 | Gift from Michael Crickmore | N/A |
| Cha-Gal4 | Gift from Michael Crickmore | N/A |
| Tim-Gal4 | Gift from Michael Young | N/A |
| TH-D4-Gal4 | Gift from Michael Crickmore | N/A |
| pros-Gal4 | Gift from Norbert Perrimon | N/A |
| CBA/CaJ mice | Jackson Laboratories | JAX 000654 |
| Oligonucleotides | ||
| CCHa1 (F-ACTGACGTCGGACAATTTGC and R-ACACGAATGTCCGTATTCCA) | ID Technologies | Custom made |
| CCHa1R (F-GTTCCAAACACCTACATTTTATCAC and R-CGGATAATGCAGTCAGCGTA) | ID Technologies | Custom made |
| CCHa2 (F-AAACAGCAACAGCAGCAAAC and R-AGGACCACGGTGCAGATAAC) | ID Technologies | Custom made |
| CCHa2R (F-CATACCCAACACATACATTCTTTC and R-GAAAGGGCGGTCAGTGTAAA) | ID Technologies | Custom made |
| rp49 (F-ATCGGTTACGGATCGAACAA and R-GACAATCT CCTTGCGCTTCT) | ID Technologies | Custom made |
| Recombinant DNA | ||
| Software and algorithms | ||
| Sleep recording Drosophila | TriKinetics | |
| Arousal threshold stimuli delivery Drosophilas | This study | |
| Sleep analysis Drosophila | Vaccaro et al 2020 | |
| Arousal threshold analysis Drosophila | This study | |
| Sleep recording mice | Pinnacle Technology Inc |
9000-K5-S; 8200-K1-iSL |
| Sleep scoring mice | Pinnacle Technology Inc | Sirenia Sleep Pro |
| Other | ||