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. 2023 May 13;12(10):1382. doi: 10.3390/cells12101382

Figure 2.

Figure 2

KV1.1 is concentrated in the pinceau in wild type and Kcna1V408A/+ cerebellum. (a) Low magnification image of cerebellar cortex folium section showing KV1.1 immunofluorescence (scale bar 100 μm). Right. High magnification confocal image showing concentrated KV1.1 immunofluorescence localized at the interface of Purkinje and granule cell layers. Right lower. Same image showing co-immunofluorescence labelling for the Purkinje cell marker calbindin (scale bar 20 μm). (b) Representative confocal sections of lamina adjacent to the Purkinje cell layer from Kcna1V408A/+ and control cerebellar cortex, labelled for KV1.1 (left, yellow) and PSD-95 (centre, magenta). Right, merged image (scale bar 20 μm). (c) Violin plot of mean KV1.1 immunofluorescence from PSD-95-outlined pinceaux (wild type n = 264, Kcna1V408/+ n = 213) from lobule V cerebellar vermal sections. Overlay. Box plot showing median and interquartile range, whiskers 1.5 * IQR from upper and lower quartiles. (d) Pinceau areas from PSD-95 immunofluorescence, * p = 0.023, wild type n = three animals, Kcna1V408A/+ n = three animals.