Table 7.
Methodological analysis modified from Delteil et al. [95].
| Item | Technique | Morphology/Quantification | Attributed PTI | Notes |
|---|---|---|---|---|
| RBCs | H&E | Intact | First 24 h | Differentiation from autolytic mechanisms by comparison with other structures |
| Lysed | From 2 to 24 days | |||
| Mixed | ||||
| Fibrin/platelets | H&E | Presence/Absence | First 72 h | - |
| Leukocytes | H&E | PMNs and/or lymphocytes | PMNs from 12 h to 17 days (peak within 48–96 h); lymphocytes from 12 h to 23 days (peak within 72–96 h) | - |
| Macrophages | CD68 immunolabeling | 200× magnification field count for semi-quantitative study | From 72 h (minimal PTI observed: 12 h) | Cells per filed proposed: 0–3 |
| Hemoglobin degradation | Perls Prussian blue stain | Detection of siderophages and/or hematoid deposits within affected tissue | From 72 h | Semi-quantitative classification as absent, early, moderate, or abundant |
| Neovascularization | H&E | Absent/present | From the 6th to the 70th day | Grades of presence: capillary proliferation, giant capillaries, arterioles |
| Fibrous organization | H&E | Absent/present | From the 2nd week | Aspects of retinal sclerosis or ON atrophy |