Table 1.

Comparison of several Aβ oligomer quantification assays according to their sensitivity level. For this purpose, the respective weight concentrations pg/mL were converted into femtomolar oligomer concentrations using the approximate molecular weights of the used calibration standards.

Reference	Assay Setup	Calibration Standard	Sensitivity [fM]
sFIDA	Single-particle analysis with fluorescence microscopy, overlapping epitopes capture: Nab228 aa1–11 detection: IC16 aa2–8 + Nab228 aa1–11	Aβ1–15 SiNaPs	LoD = 0.28
Savage et al. [30]	Single particle analysis with bead-based assay capture: 19.3 oligomer-specific detection: 82E1 aa1–16	Aβ1–42 oligomers (MW 1117 kDa)	LoD = 0.08
IBL [31]	ELISA capture: 82E1 aa1–16 detection: 24B3 oligomer-specific	E22P–Aβ40 Dimer	31.4 (N/A)
Hölttä et al. [32]	ELISA, overlapping epitopes capture and detection: 82E1 aa1–16	Dimer Aβ1–11	LLoQ = 90.9
Kasai et al. [33]	ELISA, overlapping epitopes capture and detection: Ban50 aa1–10	MAP 16-mer (lysine core)	LoD = 190
Esparza et al. [34]	Single particle analysis with bead-based assay overlapping epitopes capture and detection: HJ3.4 aa1–13	Aβ1–40Ser26Cys dimer	LLoQ = 720