Table 7.

Pepsin, trypsin, and chymotrypsin activity in the absence or presence of unsprouted (BW-0) and sprouted buckwheat (48 h, BW-48; 72 h, BW-72).

	w/o BW Extract	BW-0	BW-48	BW-72
Pepsin activity	89.44 ± 4.81b	92.41 ± 5.16b	107.41 ± 20.50ab	152.22 ± 27.76a
Trypsin activity	180.37 ± 49.83a	6.39 ± 2.03c	65.56 ± 14.35bc	82.96 ± 8.26b
Chymotrypsin activity	540.28 ± 71.10a	360.54 ± 62.22a	372.78 ± 63.79a	359.81 ± 46.65a

Pepsin, trypsin, and chymotrypsin activities are expressed as U/mg enzymes. Data are means ± SD of two different extractions and at least triplicate activity measurements. Statistical analysis was by one-way ANOVA (pepsin and trypsin: p < 0.05) with Tukey’s post hoc test (different letters indicate significant differences).