Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Roles of SH2(N) and SH2(C) domains in PLC-γ1 phosphorylation. P98 cells were transiently transfected with pcaPLC-γ1 WT (5 μg), SH2(N)* (5 μg), or SH2(C)* (15 μg). The transfected cells were stimulated for 3 min with MAb OKT3 (top) or for 5 min with 100 μM PV (bottom). Detergent-soluble proteins were immunoprecipitated with an anti-AU1 MAb, separated by SDS-PAGE, and immunoblotted with an anti-phosphotyrosine (pY) antibody. The membrane was stripped and reprobed with an anti-AU1 MAb.
