Figure 5.

Expression and release of HSP70 and HSP90 proteins in response to treatment with AgNPs-G. The Western blot was performed with (A) cell lysates and (B) supernatants from the corresponding cell line. β-actin was used as an endogenous control. Semiquantitative analysis of the immunoblot signal was performed using Image J image analysis software to provide densitometry data for each blot. Statistical analyzes were performed by ANOVA using Tukey’s test p < 0.033 (*), p < 0.002(**), p < 0.0002 (***), p ˂ 0.0001 (****), and ns (not significant p < 0.12).