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. 2023 Apr 28;14(5):1000. doi: 10.3390/genes14051000

Figure 4.

Figure 4

qPCR verification of four DEmiRNAs. (A) Shows four small RNA Seq and (B) shows the verified of the four miRNAs through qPCR. All 14 differentially expressed miRNAs were validated through qPCR the results showed that only four miRNAs (miR-184-3p, miR-276-3p, miR-87-3p, and miR-124-3p) were significantly associated with olfactory performance and memory. The relative expression data between the strong and weak olfactory performance groups (S/W) were calculated using 2−∆∆CT method. The data were analyzed using the independent sample t-test with p ≤ 0.05, n = three pooled brains per sample and eight samples from each group. The stars represents the significant level e.g., *, p ≤ 0.05 and **, p ≤ 0.005. The results of these four DEmiRNAs were consistent with small RNA-Seq.