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. 2023 May 12;24(10):8676. doi: 10.3390/ijms24108676

Figure 2.

Figure 2

TGF-β signaling reduces cell proliferation and the migration of B16F10 cells. (A) MTS assay of B16F10 caALK5 and kiALK5 cells treated with TGF-β (1 ng/mL) and/or dox (1 μg/mL) for indicated time points. The assay was performed in 10% serum. n = 3 biological replicates, mean ± S.E.M. ** p ≤ 0.01, *** p < 0.001, one way ANOVA. (B) Wound healing assay was performed using the Incucyte® system under serum-starved conditions. Prior to wounding, cells were pre-treated with the control or doxycycline (1 μg/mL) to induce the expression of caALK5 or kiALK5. During the scratch assay, cells were treated with TGF-β3 (1 ng/mL) and dox (1 μg/mL). The wounded area was followed by live cell microscopy; the relative wound width after 16 h is shown. n = 3 biological replicates, mean ± S.E.M. ns p ≥ 0.05, * p ≤ 0.05, ** p ≤ 0.01 one way ANOVA.