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. 2023 May 15;24(10):8781. doi: 10.3390/ijms24108781

Figure 1.

Figure 1

Effect of HCV infection on neutral lipid and phospholipid species in whole cell extracts. (A) HPTLC plate loaded with lipid standards and neutral lipids extracted from uninfected control (Huh7) and (JFH1) HCV-infected cells (OR—origin; MAG—monoacylglyceride; FC—free cholesterol; CL—cardiolipin; TAG—triacylglycerol; CE—cholesterol ester). (B) Quantification of FC and (C) CE. (D) HPTLC plate loaded with phospholipid standards and phospholipids extracted from uninfected control and HCV-infected cells (OR—origin; PC—phosphatidyl choline; PA—phosphatidic acid; PS—phosphatidyl serine; PI—phosphatidyl inositol; PE—phosphatidyl ethanolamine; PG—phosphatidyl glycerol). (E) Quantification of PC and (F) PE. Analysis was performed using triplicate samples from three biological replicates and normalised for protein concentration (* p < 0.05).