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. 2023 May 15;24(10):8781. doi: 10.3390/ijms24108781

Figure 2.

Figure 2

Effect of HCV infection on neutral lipid and phospholipid species in endoplasmic reticulum fractions. (A) HPTLC plate loaded with neutral lipids from ER extracted from uninfected control and HCV-infected cells (OR—origin; MAG—monoacylglycerol; FC—free cholesterol; TAG—triacylglycerol). (B) Quantification of FC in the ER of uninfected control and HCV-infected cells. (C) HPTLC plate loaded with phospholipid species from ER extracted from uninfected control and HCV-infected cells (OR—origin; PC—phosphatidyl choline; PS—phosphatidyl serine; PI—phosphatidyl inositol; PE—phosphatidyl ethanolamine). (D) Quantification of PC, (E) PE, (F) PI and (G) PS. Analysis was performed using triplicate samples from three biological replicates, normalised for protein concentration (* p < 0.05).