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. 2023 May 15;24(10):8781. doi: 10.3390/ijms24108781

Figure 3.

Figure 3

(A) Pathways responsible for the synthesis of PC involving PCYT1a and PEMT. (B) Expression in the uninfected control and HCV-infected cells of PCYT1a and (C) PEMT mRNA relative to the GapDH control, measured using real-time PCR (* p < 0.05). (D) Knock-down of PCYT1a or (F) PEMT mRNA using siRNA. (E) Intracellular HCV mRNA concentration 72 h after infection with the tissue-culture-derived virus, following an initial treatment for 48 h with 20 nM siRNA targeting PYCT1a or (G) PEMT, compared to the scrambled siRNA controls (* p < 0.05). (H) Number of infectious foci for the Huh7 cells treated with 20 nM/mL PEMT siRNA or scrambled siRNA for 48 h, then infected with the tissue-culture-derived HCV (TCID50/mL) (** p < 0.01).