Figure 6.

Rhein inhibits the growth of OC cells in a xenograft mouse model. (A) Ca9-22 cells were injected subcutaneously into the right flank of BALB/c-nu mice. After 7 days, mice were randomly allocated into 3 groups for the intraperitoneal administration of the vehicle, rhein 10 mg/kg, and rhein 50 mg/kg 3 times a week. The tumor volume was measured every 2 days, and the average tumor volumes in mice were measured. (B) Rhein significantly inhibited the growth of tumor xenografts. (C) Images of xenograft tumor volume. (D) Body weights were measured every 4 days. (E) The expression of mTOR, JNK, LC3, p62, and Bcl-2 was assessed by Western blotting. (F) H&E staining results showed no significant organ-related toxicities. (G,H) Immunohistochemical analysis of Ki67 and mTOR in xenograft tumor tissues. * p < 0.05; ** p < 0.01; *** p < 0.001.