FIG. 3.
Northern blot and Western blot analysis of the expression of the endogenous and exogenous r-PTPη gene in normal, transformed, and r-PTPη-transfected thyroid cells. (A) Northern blots. Total RNA (20 μg/lane) was extracted from PC Cl 3, PC MPSV/r-PTPη clones 1, 2, and 3, PC MPSV/pMV-7, PC MPSV, and PC MPSV/r-PTPη C/S clones 1 and 2 (left) and PC MPSV/r-PTPγ clones 1 and 2 (right) and hybridized to radiolabeled r-PTPη or GAPDH cDNAs, as indicated. (B) Western blots. Total proteins (20 μg/lane) were extracted from the cells used for panel A and hybridized with anti-r-PTPη protein, anti-v-mos, or anti-γ-tubulin antibodies. (C) Northern blots. Total RNA (20 μg/lane) was extracted from FRTL-5, FRTL-5 KiMSV/r-PTPη clones 1, 2, and 3, FRTL KIMSV, FRTL KIMSV/pMV-7, and FRTL KiMSV/r-PTPη C/S clones 1 and 2 (left) and FRTL KIMSV r-PTPη clones 1 and 2 (right) and hybridized to radiolabeled r-PTPη or GAPDH cDNAs as indicated. (D) Western blots. Total proteins (20 μg/lane) were extracted from the cells used for panel C and hybridized with anti-r-PTPη protein, anti-Ki-ras, or anti-γ-tubulin antibodies, respectively.