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. 2000 Dec;20(24):9236–9246. doi: 10.1128/mcb.20.24.9236-9246.2000

FIG. 9.

FIG. 9

Reduction of MAP kinase activity in r-PTPη-transfected thyroid cells results in up-regulation of p27Kip1 expression. (A) Western blot analysis of phosphorylated and total protein level of ERK1 and -2. Proteins extracted from normal, transformed, and r-PTPη transfected cells were separated (20 μg/lane) by SDS-PAGE, transferred to PVDF membranes, and analyzed with the indicated antibodies by Western blotting. (Top) the antibody used detected phosphorylated ERK1 and -2; (bottom) the antibody used detected total ERK1 and -2. (B) Inhibition of the MAP kinase pathway up-regulates p27Kip1 expression. (Top) Western blot analysis of p27Kip1 protein in untreated PC MPSV (lane 1) and FRTL KiMSV (lane 4) cells or in cells treated for 8 h with MEK inhibitor PD93059 (lanes 2 and 5, respectively) or solvent (dimethyl sulfoxide [DMSO]) alone (lanes 3 and 6, respectively); (middle) Western blot analysis of phosphorylated ERK1 and -2; (bottom) Western blot analysis of total ERK1 and -2.

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