FIG. 5.

EDG5 mediates inhibition of IGF I-induced p65PAK activation. (A) CHO cells and EDG cells were pretreated with S1P (0.1 μM) for 5 min or left unpretreated and then stimulated with S1P (0.1 μM) or IGF I (100 ng/ml) for a further 5 min and assayed for p65PAK activity as described in Materials and Methods. Autoradiographs and anti-myc Western blots are shown at the top. MBP, myelin basic protein. (B) EDG5 cells were transiently transfected with the empty vector or expression vectors for N¹⁷-Rac and V¹²-Rac and 48 h later were treated with S1P (0.1 μM) or IGF I (100 ng/ml) for 5 min or were left untreated. All data are means ± standard errors of three determinations. The results are representative of two experiments with similar results.