FIG. 8.

EDG5 does not mediate inhibition of IGF I-induced PI 3-kinase activation. CHO cells and EDG cells were stimulated with S1P (0.1 μM) and/or IGF I (100 ng/ml) for 5 min and assayed for PI 3-kinase activity as described in Materials and Methods. Autoradiographs of thin-layer chromatography plates and quantitated results are shown. The data are means ± standard errors of three determinations. The results are representative of two experiments with similar results. ∗, statistically significant (P < 0.05) compared to the value for nonstimulated cells analyzed as described for Table 1.