Table 5.

SPR kinetics parameters related to binding curve analyses between pm59 peptides and CD59.

(a) pm59sh
Conc. (μM)	KD (M)	ka (1/Ms)	kd (1/s)	Chi2	SE (RI)
5	4.69 × 10−7	7.26 × 103	3.40 × 10−3	0.0505	0.0505
7.5	1.24 × 10−6	4.21 × 103	5.23 × 10−3	0.0837	0.0837
10	1.64 × 10−6	3.01 × 103	4.93 × 10−3	0.1040	0.1300
15	2.39 × 10−6	2.05 × 103	4.90 × 10−3	0.1620	0.1650
20	2.61 × 10−6	1.56 × 103	4.08 × 10−3	0.1160	0.1390
25	4.13 × 10−6	1.16 × 103	4.81 × 10−3	0.0370	0.0770
30	4.46 × 10−6	1.23 × 103	5.48 × 10−3	0.2080	0.1390
Average	2.42 × 10−6	2.93 × 103	4.69 × 10−3	0.1090	0.1190
(b) pm59md
Conc. (μM)	KD (M)	ka (1/Ms)	kd (1/s)	Chi2	SE (RI)
2.5	1.38 × 10−6	5.96 × 103	8.14 × 10−3	0.0173	0.0405
5	9.02 × 10−7	7.08 × 103	6.38 × 10−3	0.0199	0.0592
10	1.09 × 10−6	4.83 × 103	5.26 × 10−3	0.0427	0.0959
20	3.10 × 10−6	2.29 × 103	7.10 × 10−3	0.0901	0.1380
30	4.39 × 10−6	1.27 × 103	6.27 × 10−3	0.0383	0.0846
40	3.88 × 10−6	1.32 × 103	5.111 × 10−3	0.0995	0.1500
50	4.90 × 10−6	0.98 × 103	4.80 × 10−3	0.1301	0.1670
average	3.13 × 10−6	2.93 × 103	5.82 × 10−3	0.0625	0.1158