Table 6.

BLI kinetics parameters related to binding curve analyses between pm59 peptides and CD59.

(a) pm59sh
Conc. (μM)	KD (M)	KD Error	ka (1/Ms)	ka Error	kdis (1/s)	kdis Error	Full X2	Full R2
5	8.646 × 10−7	2.522 × 10−8	1.133 × 103	2.252 × 101	9.798 × 10−4	2.092 × 10−5	0.0132	0.9793
10	4.983 × 10−6	8.369 × 10−8	5.236 × 102	0.814 × 101	2.609 × 10−3	1.647 × 10−5	0.0269	0.9888
20	9.921 × 10−6	1.726 × 10−7	2.939 × 102	0.473 × 101	2.916 × 10−3	1.910 × 10−5	0.0653	0.9852
30	8.446 × 10−6	1.428 × 10−7	4.510 × 102	0.658 × 101	3.809 × 10−3	3.241 × 10−5	0.2877	0.9524
40	1.496 × 10−5	2.407 × 10−7	1.966 × 102	0.281 × 101	2.941 × 10−3	2.150 × 10−5	0.2194	0.9793
50	1.59 × 10−5	2.521 × 10−7	1.779 × 102	0.244 × 101	2.832 × 10−3	2.244 × 10−5	0.4885	0.9760
average	9.182 × 10−6	1.529 × 10−7	4.627 × 102	0.786 × 101	2.681 × 10−3	2.214 × 10−5	0.1835	0.9768
(b) pm59md
Conc. ( μ M)	KD (M)	KD Error	ka (1/Ms)	ka Error	kdis (1/s)	kdis Error	Full X2	Full R 2
5	2.080 × 10−10	3.040 × 10−7	9.630 × 102	3.690 × 101	2.003 × 10−7	6.312 × 10−7	0.0096	0.9405
10	1.228 × 10−6	2.150 × 10−7	1.562 × 103	1.801 × 101	1.919 × 10−3	2.527 × 10−5	0.0170	0.9521
20	4.124 × 10−6	7.747 × 10−7	8.889 × 102	1.383 × 101	3.666 × 10−3	3.857 × 10−5	0.1905	0.8989
25	6.517 × 10−6	1.474 × 10−7	7.833 × 102	1.561 × 101	5.105 × 10−3	5.452 × 10−5	0.0690	0.9216
30	6.094 × 10−6	1.362 × 10−7	7.497 × 102	1.435 × 101	4.569 × 10−3	5.26 × 10−5	0.3972	0.8823
40	8.381 × 10−6	1.896 × 10−7	5.642 × 102	1.101 × 101	4.728 × 10−3	5.413 × 10−5	0.7459	0.8823
average	4.391 × 10−6	1.460 × 10−7	9.186 × 102	1.829 × 101	3.331 × 10−3	4.804 × 10−5	0.2839	0.9130