Figure 1.

Scheme showing the overall in vivo experimental plan. The female mice 3 months old (n = 4 in each group) were grouped and acclimatized for a week. The treatment was started with vehicle, stigmasterol, TASE, and thymol orally with a single dose per day. Mice were mated after starting the treatment from days D1 to D4 and males were separated. Treatment was continuous throughout the pregnancy and lactation stage (D1-P12). After the delivery, pups were sacrificed at P1; the brains were collected for further analysis. The mothers’ mice were fed with the TASE and thymol including the controls continuously until the pups were at P12 days and the pups’ brains were collected for analysis.