Figure 7.

TASE and thymol can rescue nocodazole-induced microtubule depolymerization. Primary hippocampal culture maintained with vehicle, positive control paclitaxel (10 nM), TASE (75 µg/mL), and thymol (10 µM) were treated with nocodazole 50 and 100 nM on DIV 2 for 2 and 24 h, respectively. On DIV 3, neurons were fixed and immunostained with α-tubulin antibody (green). (a) Immunostained images with α-tubulin antibody (green) from each treatment in 2 (50 and 100 nM nocodazole) and 24 h (50 and 100 nM nocodazole), respectively. Scale bar, 100 μm, applies to all images. (b) Mean neurite area for each treatment. NeurphologyJ plug-in and mean area (µm²) per neuron were calculated via dividing total neurites area by total soma count. Bars represent mean ± SD (n > 150 neurons). Statistical significance was compared to vehicle, vehicle, and nocodazole (### p < 0.001): * p < 0.05, and *** p< 0.001 (one- and two-way ANOVAs with Dunnett’s and Tukey’s multiple comparisons post hoc tests).