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. 2023 May 10;24(10):8565. doi: 10.3390/ijms24108565

Figure 8.

Figure 8

Effects of TASE and thymol on axonal maturation in hippocampal neurons. Neurons were grown under same culture conditions as indicated in Figure 4 for 5 days. Concentric circles of 10 μm extrapolated on neurons for Sholl analysis: (a) vehicle, (b) stigmasterol, (c) TASE, and (d) thymol treated neurons. Axonal development assessment: (e) length of axonal process. (f) Axonal collateral branching number. (g) Total length of axon collateral branch according to its order. (h) Axonal intersections point in Sholl circle. (i) Total axonal intersections in Sholl circle. (j) Axon collateral branching points in Sholl circle. (k) Total axonal collateral branching points in Sholl circle. Bars and data points represent the mean ± SD (n = 30 neurons). Statistical significance compared to vehicle: * p < 0.05, ** p < 0.01, *** p< 0.001, and ns: not significant, one- and two-way ANOVAs with Dunnett’s and Tukey’s multiple comparisons tests.