Figure 5.

Keratin staining of wild-type (A,D), non-scoliotic (B,E), and scoliotic kif7^co63/co63 (C,F) 6 wpf zebrafish (D. rerio). Keratin staining of whole cross section fish (A–C) and selective caudal vertebrae (D–F) depicting the intervertebral disk (IVD) are shown. The IVD is distinguished by vacuolated cells clearly in the wild-type and non-scoliotic kif7^co63/co63 fish, but vacuolated cells are less visible in scoliotic kif7^co63/co63. Blue: glycosaminoglycans; orange to red: prekeratin to keratin; brown: nuclei. Increased keratin/prekeratin can be seen in the IVD of scoliotic fish compared to non-scoliotic fish and wild type, as denoted by the black arrow. N = 3 zebrafish were analyzed for each group (wild type, scoliotic kif7^co63/co63, and non-scoliotic kif7^co63/co63).