FIG. 5.

TEL's PNT oligomerization domain is nonfunctional in MN1-TEL. (A) HeLa cells were transiently transfected with expression plasmids encoding HA1TELΔ53-116, HA1TEL, and TEL as indicated above the lanes. Proteins were immunoprecipitated with α-HA1. Complexes were separated on an SDS–10% polyacrylamide gel and electroblotted. The proteins were visualized by Western blot analysis with α-TEL antiserum. HA1TELΔ53-116, HA1TEL, and coprecipitating proteins are indicated by arrows on the right. A molecular mass standard is on the left. (B) COS-1 cells were (co)transfected with expression plasmids encoding HA1TEL or HA1TELΔ53-116 and MN1-TEL I, MN1-TEL II, and MN1-TEL IΔ229-1223 as indicated above the lanes. Following metabolic labeling with [³H]leucine, the proteins were immunoprecipitated with α-HA1 followed by immunoprecipitation with α-MN1 and analyzed on an SDS–polyacrylamide gel. The immunoprecipitates were visualized by autoradiography. HA1TEL, HA1TELΔ53-116, and coimmunoprecipitating proteins are indicated by arrows on the right. A molecular mass standard is on the left. +, present; −, absent.