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. 2023 May 13;24(10):8717. doi: 10.3390/ijms24108717

Figure 6.

Figure 6

Adapted population experiences lower frequency of DSBs following SN-38 exposure. (a) γH2AX foci in cells exposed to SN-38 (4 nM for 24 h). SCC7 cells are compared to the same clone after five cycles of adaptation to 4 nM SN-38. Experiment was conducted in biological triplicates. (b) Quantification of data presented in (a) of the number of foci generated 24 h post-treatment; n = 368 images were analyzed with the integrated software (refer to Section 4), images are at scale of 12.5 µm. (c) Line plot shows the number of foci remaining after 72 h of recovery from SN-38 (SCC7, 73%; T5_resistant, 87%), indicating that the rate of DSB repair is not faster in adapted cells. Data representing n = 431 images were analyzed using integrated software (refer to Section 4). Statistics were calculated using GraphPad Prism version 9.0.0, California, USA. The significance of differences was determined using an unpaired Welch’s correction two-tailed t-test (** p < 0.0021) denoted above in (b).