Figure 9.

Chromatin fractionation and covalently bound Top1. Parental line SCC1 and resistant MSC1 were treated with 40 nM of SN-38 for 6 h, and chromatin was isolated. Top1 covalently bound to chromatin was quantified using dot blot with Top1 antibody. (a) Dot blot showing the crude extract upper panel and dilution (1:2) in lower panel (for raw blot, refer to raw images). (b) Statistics were calculated for (n = 2) independent experiments using GraphPad Prism version 9.0.0, California, USA. The significance of differences was determined using unpaired Welch’s correction and two-tailed t-test (ns < 0.1234, **** p < 0.0001), as denoted.