Figure 3.
Dependence of the absorbance of the purple product (550 nm) of BSA and ABTS● on the volume of the solution of protein or protein-containing substrate. Bacto Peptone: 20 mg/mL; BSA: 10 mg/mL; egg white (10× diluted): 10.2 mg protein/mL; blood plasma (3× diluted): 20.5 mg protein/mL; ABTS●: 1.63 mM in PBS (200 μL). Reaction time: 1 h.