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. 2023 May 8;14(5):260. doi: 10.3390/jfb14050260

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of RADA16-CGFs on mineralization capability. (a) ALP staining images after culturing on the different composites for 7 days. (b) Optical microscopy images of alizarin red S staining after culturing on the different composites for 21 days. Scale bars, 100 μm. (c) Quantitative analysis of calcium nodules on the composites at a wavelength of 562 nm. *, p < 0.05; **, p < 0.005; ***, p < 0.001. (d) The gene expression of osteogenic differentiation-related proteins (Alp) of MC3T3 cells co-cultured with different composites. *, p < 0.05; **, p < 0.005.