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Fisetin decreases the inflammatory responses in LPS-mediated Raw264.7 cells. (A) Raw264.7 cells were pretreated with LPS (1 µg/mL) for 24 h and then treated with fisetin (25, 50, and 100 µM, 24 h). The expression of IL-6, IL-1β, and TNF-α were analyzed by RT-qPCR. Relative mRNA expression levels were normalized to β-actin. (B) The secretion levels of IL-6, IL-1β, and TNF-α were measured and analyzed by ELISA. * = p < 0.05. Data are representative of three experiments.
