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. 2023 May 10;9(5):550. doi: 10.3390/jof9050550

Figure 2.

Figure 2

Cell morphology of the lig4::KanMX6 S. japonicus cells was normal in complete medium (A) (YEL, incubated in a shaker at 25 °C for 4 days) (334); similar morphology was found on solid YEA medium, like the wild-type cells (B). However, the mutant strain tends to produce chains of cells on 5 mM caffeine-containing YEA (after 2 days, at 30 °C) (C), in contrast to the wild-type cells, which showed normal morphology. The chains disappeared after 7 days (D). (The cells were visualized with Nomarski optics).