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. 2023 May 16;24(10):8855. doi: 10.3390/ijms24108855

Figure 2.

Figure 2

Screening and PCR verification of recombinant strains. (A) TaPLA2 clone fragment (approximately 500 bp). M is the DL2000 DNA marker. Lanes 1–6 show PCR production of the TaPLA2 gene of YAN0802. (B) YAN0802 strain and transformant were strewed on SDA containing G418 (200 µg/mL) and cultured at 27 °C for two days. (C) RT-qPCR analysis of TaPLA2 expression in YAN0802 and transformant. (D) YAN0802 and transformant fluorescence were observed by a Zeiss fluorescence microscope. Green fluorescence was observed in the transformants. * p-value < 0.05.