. 2023 May 12;14:1141823. doi: 10.3389/fpls.2023.1141823

Table 1.

Primary oxylipins (nmol) induced in the root of soybean cultivars following inoculation with P. sojae.

Oxylipin pathway	Oxylipin	Relative abundance (nmol)
		48- h				72-h				96-h
		ORC	ORI	CRC	CRI	ORC	ORI	CRC	CRI	ORC	ORI	CRC	CRI
Lipoxygenase	9-HpODE*	45401.96^b	19235.13^c	3232.84^d	60002.61^a	44827.97^b	0.00^d	3308.41^d	0.00^d	44697.89^b	2.67^d	3168.44^d	0.00^d
	13-HOTrE*	740.27^b	779.18^b	84.27^c	1615.88^a	753.39^b	123.22^c	85.52^c	530.84^b	746.61^b	160.55^c	81.30^c	1807.62^a
	12(S)-HpETE*	0.27^b	254.80^a	0.35^b	0.37^b	0.52^b	264.63^a	0.58^b	0.37^b	0.50^b	251.89^a	0.11^b	0.31^b
	9-KOTrE*	17879.31^a	14914.77^b	764.41^e	12639.78^b	18380.18^a	3527.42^d	758.20^e	7829.71^c	18377.79^a	2315.78^e	761.83^e	15073.73^b
	13-KODE*	10103.22^b	7810.54^c	1321.30^d	16099.55^a	10042.41^b	2122.81^d	1330.11^d	5622.89^c	10042.41^b	2379.03^d	1318.21^d	16051.70^a
Allene oxide synthase	12-OPDA*	1050.02^c	2351.15^b	214.08^c	1984.07^b	1072.48^c	306.58^c	219.00^c	1358.62^c	1066.01^c	265.42^c	212.16^c	6824.43^a
Cytochrome P450	9,10-DiHODE*	54929.60^b	58177.16^b	6063.29^e	65571.54^b	55481.44^b	7123.79^e	6122.00^e	30229.56^c	55539.04^b	12852.18^d	6054.92^e	98027.91^a
	12,13-DiHOME*	2218.28^b	2755.52^b	326.19^e	2395.72^b	2032.80^b	431.09^e	323.49^e	1289.37^c	2024.81^b	759.33^d	328.39^e	3918.76^a
	16,17-EpDPE*	0.02^d	5161.15^c	0.52^d	0.34^d	0.45^d	6357.31^b	0.18^d	0.53^d	0.25^d	7519.81^a	0.72^d	0.30^d

Summary of primary oxylipin (nmol) in the roots of both soybean cultivars. Data presented are means ± standard errors for four sample replicates. Means in the same row accompanied by different superscripts represent significance differences (*) between the treatments, consisting of susceptible root control (ORC) and susceptible root inoculated (ORI); and tolerant root control (CRC) and tolerant root inoculated (CRI) from 10-day old plants following a post-inoculation period of 48 h, 72 h or 96 h. Significance level assessed at α < 0.05. The oxylipins detected were 10(E), 12(E)-9-hydroperoxyoctadeca-10,12-dienoic acid (9-HpODE), 10(E),12(Z), 13S-hydroxy-9(Z),11(E),15(Z)-octadecatrienoic acid (13-HOTrE), and 12S-hydroperoxy-5(Z),8(Z),10(E),14(Z)-eicosatetraenoic acid (12(S)-HpETE), 15(Z)-9-oxo-octadecatrienoic acid (9-KOTrE) and (9Z,11E)-13-Oxo-9,11-octadecadienoic acid (13-KODE), 12-oxophytodienoic acid (12-OPDA), (12Z,15Z)-9,10-dihydroxyoctadeca-12,15-dienoic acid (9, 10-DiHODE), (Z)-12,13-dihydroxyoctadec-9-enoic acid (12,13-DiHOME), and (4Z,7Z,10Z,13Z)-15-[3-[(Z)-pent-2-enyl]oxiran-2-yl]pentadeca-4,7,10,13-tetraenoic acid (16,17-EpDPE).