Table 3.
Lipid class | Oxidized glycerolipids | Relative abundance (nmol) | |||
---|---|---|---|---|---|
ORC | ORI | CRC | CRI | ||
Phosphatidylcholine | PC36:6+2O* | 9.65 ± 0.88c | 12.47 ± 0.61b | 0.00 ± 00d | 18.02 ± 0.10a |
PC36:5+2O* | 20.10 ± 0.78b | 16.58 ± 0.46c | 0.00 ± 00d | 23.34 ± 0.21a | |
Phosphatidylethanolamine | PE38:6+O* | 15.39 ± 0.28b | 25.23 ± 0.45a | 0.00 ± 00d | 8.58 ± 0.18c |
PE38:6+2O* | 9.65 ± 0.88b | 11.97 ± 0.39a | 0.00 ± 00d | 8.79 ± 0.40c | |
Phosphatidic acid | PA34:3+O* | 10.67 ± 0.25a | 6.48 ± 0.38b | 0.00 ± 00c | 10.85 ± 0.26a |
Phosphatidylinositol | PI28:3+2O* | 3.60 ± 0.21a | 3.06 ± 0.13a | 0.00 ± 00b | 0.36 ± 0.02b |
Triacylglycerol | TG50:3+O* | 7.63 ± 0.17b | 12.81 ± 0.57a | 0.38 ± 0.16d | 6.49 ± 0.23c |
TG52:6+O* | 5.33 ± 0.16a | 3.97 ± 0.32b | 0.00 ± 00d | 0.75 ± 0.05c | |
TG54:2+O* | 2.52 ± 0.20b | 1.63 ± 0.60c | 0.12 ± 0.01d | 3.51 ± 0.20a | |
TG54:8+2O* | 1.34 ± 0.53d | 5.05 ± 0.25c | 37.92 ± 0.23a | 19.63 ± 0.15b | |
TG54:8+3O* | 1.55 ± 0.41b | 2.03 ± 0.50a | 0.31 ± 0.01c | 2.12 ± 0.04a | |
TG54:6+Ox* | 0.38 ± 0.20d | 3.02 ± 0.13c | 51.45 ± 0.29a | 24.56 ± 0.41b |
Summary of oxidized glycerolipids (nmol) in the roots of both soybean cultivars. Data represented are means ± standard errors for four sample replicates. Means in the same row accompanied by different superscripts represent significance differences (*) between the treatments, consisting of susceptible root control (ORC) and susceptible root inoculated (ORI); and tolerant root control (CRC) and tolerant root inoculated (CRI) from 10-day old plants. Significance level assessed at α = 0.05. The oxylipins detected were oxidized phosphatidylcholine (Ox-PC), oxidized phosphatidylethanolamine (Ox-PE), oxidized phosphatidic acid (Ox-PA), oxidized phosphatidylinositol (Ox-PI), and oxidized triacylglycerol (Ox-TG), O = monoxide, 2O = dioxide, 3O = trioxide and Ox = oxidized.