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. 2023 May 16;9(5):576. doi: 10.3390/jof9050576

Table 2.

Biofilm and extracellular matrix components of CaS biofilms after ZER treatments.

Component Groups 5 min 10 min 20 min
Mean Standard Deviation Mean Standard Deviation Mean Standard Deviation
CFU/mL Control 8.33 × 105 Aa 1.71 × 105 1.16 × 106 Ba 1.24 × 105 2.17 × 106 Ca 2.77 × 105
ZER-128 1.63 × 105 Ab 4.96 × 104 2.40 × 105 Bb 7.82 × 104 4.13 × 105 Cb 1.30 × 105
ZER-256 1.10 × 105 Ac 3.02 × 104 1.73 × 105 Bc 5.21 × 104 1.73 × 105 Bc 3.94 × 104
Dry-Weight (mg) Control 5.733 Aa 0.939 5.266 Aa 0.695 5.566 Aa 0.752
ZER-128 3.400 Ab 0.898 3.066 Ab 0.574 3.383 Ab 0.829
ZER-256 2.349 Ac 0.383 2.166 Ac 0.389 2.450 Ac 0.566
Insoluble
Dry-weight (mg)		 Control 1.200 Aa 0.062 1.198 Aa 0.053 1.128 Aa 0.115
ZER-128 1.119 Ab 0.099 1.096 Ab 0.085 1.110 Aa 0.055
ZER-256 0.663 Ac 0.035 0.641 Ac 0.069 0.624 Ab 0.060
Soluble proteins (µg) Control 0.024 Aa 0.002 0.022 Ba 0.001 0.021 Ba 0.001
ZER-128 0.023 Aab 0.002 0.022 ABa 0.001 0.021 Ba 0.001
ZER-256 0.022 Ab 0.002 0.022 Aa 0.001 0.021 Aa 0.001
Insoluble proteins (µg) Control 0.010 Aa 0.001 0.010 ABa 0.002 0.011 Ba 0.002
ZER-128 0.010 Aa 0.001 0.010 Aa 0.001 0.011 Aa 0.002
ZER-256 0.010 Aa 0.001 0.010 Aa 0.001 0.009 Ab 0.001
WSP (µg) Control 0.090 Aa 0.009 0.092 Aa 0.014 0.091 Aa 0.004
ZER-128 0.083 Aa 0.006 0.071 Bb 0.017 0.069 Bb 0.017
ZER-256 0.033 Ab 0.003 0.032 Ac 0.002 0.031 Ac 0.001
ASP (µg) Control 0.100 Aa 0.008 0.101 Aa 0.009 0.100 Aa 0.006
ZER-128 0.100 Aa 0.012 0.096 Aab 0.002 0.094 Aab 0.009
ZER-256 0.092 Aa 0.010 0.094 Ab 0.002 0.092 Ab 0.007
eDNA (ng) Control 35.904 ABa 2.99 35.189 Aa 4.52 39.356 Ba 6.05
ZER-128 22.452 Ab 4.00 22.167 Ab 3.82 21.669 Ab 5.27
ZER-256 14.903 Ac 1.05 11.720 ABc 2.54 8.541 Bc 1.78

The data are shown as average and standard deviation (n = 12): viable colonies counting (CFU/mL); Dry-Weight (mg); Insoluble Dry-Weight (mg); Soluble proteins (µg); Insoluble proteins (µg); Water-soluble polysaccharides (WSP; µg); Alkali-soluble polysaccharides (ASP; µg); extracellular DNA (eDNA; ng). Capital letters show the comparison among the times (lines) and lowercase letters show the comparison among the concentrations (columns). Unequal letters indicate significant statistical differences (p < 0.05).